Part:BBa_K1679028:Design

T5 promoter + RBS + 6x His Tag + ftnA + λ T0 Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 133
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We created this device for expression of ftnA in E.coli for a long time. T5 promoter is weaker than T7 promoter, so that it can exclude stress responses and adapt to long-time expression. The 6x His tag is design for Ni-chelating affinity chromatography. This device was adapted from the cassette for express ftnA on PQE_80L and similar to it.

Fig.1. FtnA on PQE_80L.

Fig.2. FtnA on our device.

Source

Synthesized by Genewiz.

References

[1]Smith J L. The physiological role of ferritin-like compounds in bacteria[J]. Critical reviews in microbiology, 2004, 30(3): 173-185.

[2] Papaefthymiou G C, Viescas A J, Devlin E, et al. Electronic and magnetic characterization of in vivo produced vs. in vitro reconstituted horse spleen ferritin[C]//MRS Proceedings. Cambridge University Press, 2007, 1056: 1056-HH03-27.

[3] Stanley S A, Sauer J, Kane R S, et al. Remote regulation of glucose homeostasis in mice using genetically encoded nanoparticles[J]. Nature medicine, 2015, 21(1): 92-98.